Promotor | : | Prof. Dr. Ir. Siti Subandyah, M.Agr.Sc | |
Co-Promotor 1 | : | Prof. Dr. drh. Wayan Tunas Artama | |
Co-Promotor 2 | : | Dr. Windi Muziasari | |
Dissertation Title | : |
Antibiotic Resistance Gene Monitoring in the Agricultural Environment in Java, Indonesia |
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Abstract | : | The use of antimicrobial agent in livestock farm has been a common strategy for several purposes including bacterial, protozoal, and fungal infection treatment, and growth promotor. Antibiotics, the most common antimicrobial agent use in livestock, are commonly mixed with the feeds for bacterial infection treatment or as a growth promotor. This accelerates the emerging of antibiotic resistant bacteria in the animal’s gut microbiomes. When the animal manures are applied to the soil, it is not only antibiotic compounds, but also considerable numbers of antibiotic-resistant bacteria, antibiotic resistance genes (ARGs), and associated mobile genetic elements (MGEs) are introduced to soil. Several kinds of antimicrobial agents also used for managing the plant health including fungicides, bactericides (antibacterials), insecticides, and pesticides. This phenomenon can drive those antimicrobial residues from animal and plant to agriculture sector. This study is designed to characterize the presence and the abundance of targeted ARGs and MGEs, monitor the gene distribution, and study the gene’s prevalence in agricultural environment using culture independent and molecular microbiology technology including conventional PCR, qPCR, a high throughput qPCR (HTqPCR), and the SmartChip qPCR system. Three kinds of samples including animal feces, animal manure, and plant cultivating soil are collected from several agricultural farms in Yogyakarta, consisting of chili, shallot, and rice field. The study will be divided into three stages: (1) profile of the ARGs and MGEs in Integrated Farming Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta and Java, Indonesia (2) design a primer set to target the important ARGs and MGEs according to the first stage result through an in silico analysis and (3) study the prevalence of ARGs and MGEs in Yogyakarta agricultural environment based on designed and controlled farming systems in Bantul district, Yogyakarta. | |
Keywords | : | antibiotic resistance gene, mobile genetics element, feces, manure, soil | |
Publications and Seminars | : | World Microbe Forum, 20-24 June 2021, Online Worldwide | |
The 6th International Symposium Association of Japan-Indonesia Veterinary Education 2022 (11 Febtuary 2022) | |||
: | putu.cri.d@ugm.ac.id |
Promotor | : | Ir. Donny Widianto, Ph.D. | |
Co-Promotor 1 | : | Prof. Ir. Irfan Dwidya Prijambada, Ph.D. | |
Dissertation Title | : |
Exploration of Fructophilic Yeast and Its Utilization to Improve Ethanol Fermentation Efficiency from Sweet Sorghum Juice |
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Abstract | : | In biofuel production, using raw materials that do not conflict with the food supply and are easy to cultivate, is expected to be a solution to the provision of sustainable raw materials and can reduce production prices. Sweet sorghum can meet feedstock requirements for bioethanol production as it contains relatively high glucose and fructose content. During ethanol fermentation, yeast tends to use glucose first, compared to fructose. As a result, when other nutrients are depleted, fructose residues remain at the end of fermentation. This condition causes fermentation to get sluggish and stuck. Fructophilic yeast was required to solve the fermentation problem. The fructophilic tendency is linked to hexose transporters that transport glucose and fructose into cells. This study aimed to isolate and characterize fructophilic yeasts, investigate the correlation between the Hxt1, Hxt3, and Fsy1 transporters and their fructophilic behavior, and evaluate the capacity of these yeasts to enhance the fermentation efficiency of sweet sorghum juice into ethanol. | |
Keywords | : | Yeast, fructophilic, sweet sorghum, bioethanol | |
Publications and Seminars | : |
International Conference for Green Science and Technology, (provided online by Shizuoka University ), 21- 22 September 2021 |
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11th International Seminar of Indonesian Society for Microbiology (11th ISISM), (online, 16-17 November, 2021) | |||
Medhina Magdalena, Candra Rahmani Priyambada, Donny Widianto, Sri Wedhastri, Irfan Dwidya Prijambada. Yeast Diversity in Honey Produced by Wild Honeybees at Different Elevations. Baghdad Science Journal (Accepted) | |||
: | medhinamagdalena2018@mail.ugm.ac.id |
Promotor | : | Prof. Dr. Rarastoeti Pratiwi, M.Sc. | |
Co-Promotor 1 | : | Prof. Dr. Abdul Salam Sofro | |
Co-Promotor 2 | : | Dr. dr. Teguh Triyono, M.Kes, Sp.PK (K) | |
Dissertation Title | : | Molecular Basic of ABO Blood Group System in Indonesia | |
Abstract | : | Among the 45 human blood group systems reported so far, the ABO system was the most important. In the field of transfusion and transplant medicine, the accuracy of ABO blood group typing was clinically important. To characterize ABO blood group status, the gold standard remains serological testing. As an alternative and/or complementary approach to the standard blood group serological typing, genotyping has emerged in immunohematology laboratories for years. To this aim, the molecular determinants driving phenotype variability must be reported on a population-specific basis, to guarantee an optimal identification of the variations by molecular typing. Although widely studied in the Caucasian and East-Asian populations, but the was no molecular data have been generated in Indonesia. The purpose of this research was to study the ABO system blood group polymorphisms in the Indonesian population, especially related to the molecular study of the ABO gene. The study begins with standart serological identification to find unusual variants. These sample then be identified by PCR-ASP and PCR-sequencing techniques. Correlation between phenotypes and genotypes were analyzed by functional genetic studies. Allele and genotype frequencies were also estimated. Then new novel population-specific variants/clusters could be determined. | |
Keywords | : | ABO Blood Group, ABO Distribution, Genotyping, Indonesia | |
Publications and Seminars | : | myHAM UMY, 11th May 2024 (The 1st International Conference Muhammadiyah Yogyakarta – Hospital & Healthcare Management) | |
ICOPH-TCD UNDIP, 30th-31st July 2024 (International Conference on Public Health for Tropical and Coastal Development) | |||
Distribution of ABO and Rhesus D antigen expression in Yogyakarta, Java Island: a pioneer large-scale study in Indonesia. BMC RESEARCH NOTE (peer review) | |||
: | hasnafadlilatul@gmail.com |
Promotor | : | Prof. Dr. Abdul Rohman, S. F., M. Si., Apt. | |
Co-Promotor 1 | : | Dr. Tri Rini Nuringtyas, S. Si., M. Sc. | |
Co-Promotor 2 | : | Dr. Djoko Santosa, S. Si., M. Si. | |
Dissertation Title | : |
In Vitro Culture of Red Ginger (Zingiber officinalle var. rubrum) with Fusarium oxysporum's elicitation as Anti-Salmonella sp. |
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Abstract | : |
The potential of red ginger as a herbal medicine is supported by the content of secondary metabolite compounds which have been proven to be important in the health sector. The high demand for red ginger in society leads to the need of an appropriate cultivation methods to produce red ginger with optimal secondary metabolite content. Red ginger can be cultured in laboratory using explants in the form of shoots from the rhizome. Increasing the production of secondary metabolite compounds through in vitro culture can be optimized by adding elicitors. Elicitation of in vitro cultures is carried out to increase the production of secondary metabolite compounds by induction of stress through abiotic and biotic factors. One of the endogenous fungi of red ginger in nature is Fusarium oxysporum, so this type has the potential to be used as an elicitor in in vitro cultures. This study aims to identify differences in metabolites produced using a metabolomics approach and genes expressed using a transcriptomics approach in red ginger resulting from in vitro culture with and without elicitors and how their activity is as an anti-Salmonella sp. so that its potential as a herbal medicine can be optimized. |
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Keywords | : | elicitor, Fusarium oxysporum; metabolomic; transcriptomic; Zingiber officinale | |
Publications and Seminars | : | The 6th International Seminar on Agribusiness, Semarang, July 26th 2023 | |
Dyah Ayu Widyastuti, Tri Rini Nuringtyas, Djoko Santosa, Abdul Rohman. A perspective on the in vitro tissue culture of red ginger and its impact on antioxidant activity. Food Research (Accepted) and Dyah Ayu Widyastuti, Djoko Santosa, Tri Rini Nuringtyas, Siti Fatimah, Abdul Rohman. Total phenolic and flavonoid contents and antioxidant activity of red ginger (Zingiber officinale var. rubrum)’s rhizomes from seven different regions in the island of Java Indonesia. Food Research (Under Review) |
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dyah.ayu.widyastuti@mail.ugm.ac.id |